Sequential feast-famine process for polyhydroxyalkanoates production by mixed methanotrophic culture under different carbon supply and pH control strategies

Aleksandra Gęsicka; Natalia Gutowska; Sivasankar Palaniappan; Piotr Oleśkowicz-Popiel; Mateusz Łężyk

doi:10.1016/j.jece.2024.115221

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Title

Sequential feast-famine process for polyhydroxyalkanoates production by mixed methanotrophic culture under different carbon supply and pH control strategies

Authors

Aleksandra Gęsicka (WIŚiE) ^{[ 1 ][ 2.10 ][ SzD ]}
Natalia Gutowska (WIŚiE) ^{[ 2 ][ 2.10 ][ P ]}
Sivasankar Palaniappan (WIŚiE) ^{[ 2 ][ 2.10 ][ P ]}
Piotr Oleśkowicz-Popiel (WIŚiE) ^{[ 2 ][ 2.10 ][ P ]}
Mateusz Łężyk (WIŚiE) ^{[ 2 ][ 2.10 ][ P ]}

^{[ 1 ]} Wydział Inżynierii Środowiska i Energetyki, Politechnika Poznańska | ^{[ 2 ]} Instytut Inżynierii Środowiska i Instalacji Budowlanych, Wydział Inżynierii Środowiska i Energetyki, Politechnika Poznańska | ^{[ SzD ]} doctoral school student | ^{[ P ]} employee

Scientific discipline (Law 2.0)

[2.10] Environmental engineering, mining and energy

Year of publication

2025

Published in

Journal of Environmental Chemical Engineering

Journal year: 2025 | Journal volume: vol. 13 | Journal number: iss. 1

Article type

scientific article

Publication language

english

Keywords

EN

Polyhydroxyalkanoates
PHBV
Methanotrophs
Cosubstrates
Feast-famine regime

Abstract

EN Microbial synthesis of biopolymers from methane presents an environmentally friendly alternative to traditional plastics production. The production of polyhydroxyalkanoates (PHAs) from methane was assessed, along with the impact of various cosubstrates on PHA copolymer composition. Valeric acid, as the most effective cosubstrate for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) accumulation, was used for the first time to study PHAs production from methane by mixed methanotrophic culture in a sequential feast-famine process. The influence of various gas flow rates, cosubstrate feeding strategies, and pH on culture growth and PHA accumulation was investigated. A high 3-hydroxyvalerate fraction of approximately 60 mol% at 0.1 standard litre per minute (slpm) and 40 mol% at 0.2 slpm was observed. The maximum biomass concentration of 2.25 g/L and a PHA concentration of about 0.5 g/L was achieved. A pH change led to a microbial shift from Methylocystis hirsuta to Cupriavidus metallidurans, without impacting the PHA accumulation capacity.

Date of online publication

25.12.2024

Pages (from - to)

115221-1 - 115221-10

DOI

10.1016/j.jece.2024.115221

URL

https://www.sciencedirect.com/science/article/pii/S2213343724033530?via%3Dihub

Comments

Article Number: 115221

Ministry points / journal

100