Title
Concentration-dependent disturbances of digestive functions in house cricket (Insecta: Orthoptera) exposed to GO-AgNP composite
Authors
[ 1 ] Instytut Fizyki, Wydział Inżynierii Materiałowej i Fizyki Technicznej, Politechnika Poznańska | [ P ] employee
Scientific discipline (Law 2.0)
Year of publication
2025
Published in
Article type
scientific article
Publication language
english
Keywords
EN
- Acheta domesticus
- Food consumption and assimilation
- Dead cells and ROS + cells
- Gut histology
- Digestive enzymes
Abstract
EN This study investigates the effects of graphene oxide (GO) and silver nanoparticle (AgNP) composite (GO-AgNP) on the digestive physiology and gut ultrastructure of Acheta domesticus (house cricket) during extended exposure. Various concentrations of GO-AgNP were tested to assess their impact on food consumption, assimilation, cell status (Dead Cells and ROS + cells), gut enzyme activity, and structural damage to gut cells. Concentration and exposure time had significant effects on oxidative stress, enzyme activity, and gut cell structure. The applied composite reduced cumulative food consumption and assimilation efficiency. Enzyme assays showed that lower concentrations enhanced carbohydrate-degrading enzyme activity, while higher concentrations inhibited protease activity. Histological analysis revealed structural damage to gut epithelial cells and signs of autophagy or necrosis at higher concentrations. These results suggest that GO and AgNPs contribute to oxidative stress, cell cycle disruption, and apoptosis, with AgNPs having a potentially stronger effect than GO. The disturbed enzyme activity may result from conformational changes caused by nanoparticle agglomeration. These findings underline potential risks associated with the environmental or agricultural use of GO-AgNP composites.
Date of online publication
13.04.2025
Pages (from - to)
12699-1 - 12699-17
License type
CC BY (attribution alone)
Open Access Mode
open journal
Open Access Text Version
final published version
Date of Open Access to the publication
at the time of publication
Ministry points / journal
140
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