2025

Journal of Drug Delivery Science and Technology

Journal year: 2025 | Journal volume: vol. 111

scientific article

english

EN The aim of this study was to develop hydrogel-based 3D-printed scaffolds incorporating asiaticoside-rich extract and varying concentrations of chitosan (CS) for enhanced wound healing applications. The primary objective was to optimize the scaffold formulation to achieve desirable printability, mechanical stability, and biological performance. The scaffold containing 1 % of Centella asiatica extract, 2 % of gelatin and 3.5 % of CS (1Ex/2Gel/3.5CS) emerged as the optimal composition among the tested formulations. This version demonstrated superior printability, shape fidelity, and structural integrity. Rheological analysis indicated that increasing CS concentration elevated the viscosity, which improved the mechanical strength and stability of the scaffold but slightly reduced its fluidity. Spectroscopic (Fourier Transform Infrared Spectroscopy with Attenuated Total Reflectance, ATR-FTIR) and X-ray diffraction (XRPD) analyses confirmed molecular interactions between asiaticoside and chitosan, contributing to the enhanced physical stability of the scaffold. Drug release studies showed a controlled and sustained release of asiaticoside, with approximately 80 % released from the optimal formulation within 24 h, supporting prolonged therapeutic action. The scaffold also exhibited high swelling capacity and significant anti-inflammatory activity. Biocompatibility assays confirmed its support for fibroblast migration and proliferation, leading to up to 99.9 % wound closure in vitro. Overall, these results demonstrate that the optimized 3D-printed scaffold has strong potential for tissue engineering and wound healing applications.

107143 - 107143

10.1016/j.jddst.2025.107143

https://www.webofscience.com/wos/woscc/full-record/WOS:001506930400002

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