Title
Exploration of Sapogenins as Promising Lead Compounds in the Development of Novel Antimicrobial Agents
Authors
[ 1 ] Wydział Technologii Chemicznej, Politechnika Poznańska | [ 2 ] Instytut Technologii i Inżynierii Chemicznej, Wydział Technologii Chemicznej, Politechnika Poznańska | [ SzD ] doctoral school student | [ P ] employee
Scientific discipline (Law 2.0)
Year of publication
2025
Published in
Article type
scientific article
Publication language
english
Keywords
EN
- bacterial metabolism
- Gram-negative bacteria
- liposomes
- sapogenins
- toxicity
Abstract
EN Sapogenins, the aglycone moieties of saponins, have garnered interest for their potential as antimicrobial agents due to their membrane-disrupting properties. This study investigates a selection of natural and semi-synthetic derivatives of two key sapogenins, diosgenin and tigogenin, to evaluate their antimicrobial efficacy against Escherichia coli and Pseudomonas aeruginosa. A series of derivatives with modifications at the C-3 position were synthesised and tested for their effects on bacterial growth, carbon metabolism, and membrane disruption. Results from microbial growth assays identified pseudo-diosgenin and (25R)-5α-spirostan-3β-yl l-glutamate as themost effective inhibitors of E. coli, while P. aeruginosa showed high sensitivity to (25R)-spirost-5-en-3β-yl pyridine-3-carboxylate and pseudo-diosgenin. The EcoPlate assays demonstrated compound specific inhibition of bacterial metabolism, particularly by diosgenin ((25R)-spirost-5-en-3β-yl (E)-3-(3,4-dihydroxyphenyl)acrylate) and pseudodiosgenin ((25R)-furost-5-en-3β-acetoxy-26-ol), highlighting disruptions in key pathways such as carbohydrate and phenolic compounds metabolism. These findings underscore the potential of sapogenins, particularly modified derivatives, as promising leads in the development of novel antimicrobial therapies.
Date of online publication
13.08.2025
Pages (from - to)
e01569-1 - e01569-12
Comments
Article number: e01569
License type
CC BY (attribution alone)
Open Access Mode
czasopismo hybrydowe
Open Access Text Version
final published version
Release date
13.08.2025
Date of Open Access to the publication
at the time of publication
Full text of article
Access level to full text
public
Ministry points / journal
70
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