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Dark-fermentative hydrogen production from synthetic lignocellulose hydrolysate by a mixed bacterial culture: The relationship between hydraulic retention time and pH conditions


[ 1 ] Instytut Inżynierii Środowiska i Instalacji Budowlanych, Wydział Inżynierii Środowiska i Energetyki, Politechnika Poznańska | [ P ] employee

Scientific discipline (Law 2.0)

[2.9] Environmental engineering, mining and energy

Year of publication


Published in

Bioresource Technology

Journal year: 2022 | Journal volume: vol. 358

Article type

scientific article

Publication language


  • Biohydrogen
  • Pentose
  • Hexose
  • Dark fermentation
  • Continuous stirred tank reactor

EN This study assessed the effect of hydraulic retention time (HRT) ranging from 24 to 3 h on continuous dark-fermentative H2 production in four bioreactors operated at pH 5.0, 5.5, 6.0 and 6.5. A mixture of cellobiose, xylose and arabinose was used as the substrate. The highest hydrogen production rate between HRTs of 24 and 12 h was observed at pH 6.5, while at HRT below 12 h at pH 6.0. At a HRT of 3 h it reached 11.4 L H2/L-d. Thus, the optimum pH for H2 production depends on the HRT. The highest sugar utilization was obtained at pH 6.0 and 6.5 and decreased in the following order: cellobiose > xylose > arabinose. The pH conditions, in contrast to HRT, were found to have a significant influence on the bacterial composition. Low diversity in bacterial culture dominated by the Clostridium genus allows for stable and high H2 production performance.

Pages (from - to)

127309-1 - 127309-10





Article Number: 127309

License type

CC BY-NC-ND (attribution - noncommercial - no derivatives)

Open Access Mode

czasopismo hybrydowe

Open Access Text Version

final published version

Date of Open Access to the publication

in press

Points of MNiSW / journal


Impact Factor

9.642 [List 2020]

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