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Article

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Title

In silico and in vitro analysis of the impact of single substitutions within EXO-motifs on Hsa-MiR-1246 intercellular transfer in breast cancer cell

Authors

[ 1 ] Instytut Informatyki, Wydział Informatyki i Telekomunikacji, Politechnika Poznańska | [ P ] employee | [ S ] student

Scientific discipline (Law 2.0)

[2.3] Information and communication technology

Year of publication

2023

Published in

Journal of Applied Genetics

Journal year: 2023 | Journal volume: vol. 64 | Journal number: iss. 1

Article type

scientific article

Publication language

english

Keywords
EN
  • Breast cancer
  • EXO-motifs
  • Extracellular vesicles
  • MicroRNA
  • RNA structure prediction
Abstract

EN MiR-1246 has recently gained much attention and many studies have shown its oncogenic role in colorectal, breast, lung, and ovarian cancers. However, miR-1246 processing, stability, and mechanisms directing miR-1246 into neighbor cells remain still unclear. In this study, we aimed to determine the role of single-nucleotide substitutions within short exosome sorting motifs — so-called EXO-motifs: GGAG and GCAG present in miR-1246 sequence on its intracellular stability and extracellular transfer. We applied in silico methods such as 2D and 3D structure analysis and modeling of protein interactions. We also performed in vitro validation through the transfection of fluorescently labeled miRNA to MDA-MB-231 cells, which we analyzed by flow cytometry and fluorescent microscopy. Our results suggest that nucleotides alterations that disturbed miR-1246 EXO-motifs were able to modulate miRNA-1246 stability and its transfer level to the neighboring cells, suggesting that the molecular mechanism of RNA stability and intercellular transfer can be closely related.

Date of online publication

17.11.2022

Pages (from - to)

105 - 124

DOI

10.1007/s13353-022-00730-y

URL

https://link.springer.com/article/10.1007/s13353-022-00730-y

License type

CC BY (attribution alone)

Open Access Mode

czasopismo hybrydowe

Open Access Text Version

final published version

Date of Open Access to the publication

in press

Ministry points / journal

140

Impact Factor

2,4 [List 2022]

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