Depending on the amount of data to process, file generation may take longer.

If it takes too long to generate, you can limit the data by, for example, reducing the range of years.


Download BibTeX


Continuous dark-photo fermentative H2 production from synthetic lignocellulose hydrolysate with different photoheterotrophic cultures: Sequential vs. co-culture processes


[ 1 ] Instytut Inżynierii Środowiska i Instalacji Budowlanych, Wydział Inżynierii Środowiska i Energetyki, Politechnika Poznańska | [ P ] employee

Scientific discipline (Law 2.0)

[2.10] Environmental engineering, mining and energy

Year of publication


Published in


Journal year: 2024 | Journal volume: vol. 290

Article type

scientific article

Publication language


  • Biohydrogen
  • Integrated process
  • Photo-fermentation
  • Dark fermentation
  • Co-fermentation

EN Integration of dark and photo-fermentation is a promising strategy to efficiently produce renewable hydrogen from different types of waste biomass. In this study, sequential and co-culture dark-photo fermentations were evaluated and compared during continuous operation using synthetic lignocellulose hydrolysate as a substrate. Mixed dark-fermentative bacterial consortium was combined with two different photo-fermentative bacterial cultures: pure culture of Rhodobacter sphaeroides (RSC) and mixed photoheterotrophic consortium (MPC). The sequential process showed higher total H2 yields compared to co-culture fermentation for both RSC and MPC. The highest yield of 4.15 mol H2/molsugar was obtained for the sequential process with RSC at an HRT of 3.5 days and pH 7.5. For the co-culture process, the yield reached 2.88 mol H2/molsugar at pH 7.0 and an HRT of 3.5 days. Complete sugar utilization was observed for dark fermentation. RSC preferred acetate as a carbon source, while MPC preferred butyrate. Photoheterotrophic bacteria, Rhodopseudomonas pseudopalustris and Rhodoplanes piscinae, present in the MPC inoculum were outcompeted by undesirable microorganisms during the continuous process, resulting in low HPR. On the other hand, in both sequential and co-culture processes, a high relative abundance of R. sphaeroides (>40%) was observed in the RSC, indicating effective cooperation between dark and photo bacteria.

Pages (from - to)

130105-1 - 130105-11





Article Number: 130105

Ministry points / journal


Impact Factor

8,9 [List 2022]

This website uses cookies to remember the authenticated session of the user. For more information, read about Cookies and Privacy Policy.