Exogenous production of cold-active cellulase from polar Nocardiopsis sp. with increased cellulose hydrolysis efficiency
[ 1 ] Instytut Inżynierii Środowiska i Instalacji Budowlanych, Wydział Inżynierii Środowiska i Energetyki, Politechnika Poznańska | [ P ] employee
2022
scientific article
english
- cold-active enzyme
- cellulase
- psychrophiles
- cellulose hydrolysis
- actinobacteria
- Southern Ocean
EN The present work was designed to isolate and characterise the actinobacteria in the Polar Front region of the Southern Ocean waters and species of Nocardiopsis and Streptomyces were identifed. Among those, the psychrophilic actinobacterium, Nocardiopsis dassonvillei PSY13 was found to have good cellulolytic activity and it was further studied for the production and characterisation of cold-active cellulase enzyme. The latter was found to have a specifc activity of 6.36 U/mg and a molar mass of 48 kDa with a 22.9-fold purifcation and 5% recovery at an optimum pH of 7.5 and a temperature of 10 °C. Given the importance of psychrophilic actinobacteria, N. dassonvillei PSY13 can be further exploited for its benefts, meaning that the Southern Ocean harbours biotechnologically important microorganisms that can be further explored for versatile biotechnological and industrial applications.
25.03.2022
218-1 - 218-9
Article Number: 218
70
2,8