Antioxidant activity of flavonoids of different polarity, assayed by modified ABTS cation radical decolorization and EPR technique
[ 1 ] Instytut Fizyki, Wydział Fizyki Technicznej, Politechnika Poznańska | [ D ] phd student | [ P ] employee
2010
scientific article
english
- 7-O-β-[2-O-feruloyl-β-glucuronopyranosyl (1→2) glucuronopyranoside] (tricine)
- 4'-methoxy-5
- 7-dihydroxyflavone 6-C-β-glucopyranoside (isocytisoside)
- I 3' II 8 biapigenine (amentoflavone)
- all-trans β-carotene
- ABTS cation radical
- TEAC
- EPR technique
- free radicals
- oxidation
EN Modified ABTS cation radical decolorization assay and EPR technique were applied to screen the antioxidant activity of three flavonoids with different polarity: 7-O-β-[2-O-feruloyl-β-glucuronopyranosyl (1→2) glucuronopyranoside] (tricine), 4'-methoxy-5,7-dihydroxyflavone 6-C-β-glucopyranoside (isocytisoside) and I 3' II 8 biapigenine (amentoflavone), with nonpolar all-trans β-carotene used as standard carotenoid molecule. The ABTS [2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid] cation radical decolorization assay was modified as follows: (1) measurements extended up to 8 days after preparation, (2) method adapted for flavonoids with different polarity and β-carotene, (3) concentrations in the 0.01-10 μM range of both trolox and antioxidants in order to use the same experimental conditions for both this technique and EPR measurement.
97 - 104
publisher's website
final published version
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