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Article

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Title

Determination of L‑selectin in blood plasma using DNA aptamer–based surface‑enhanced Raman spectroscopy assay

Authors

[ 1 ] Instytut Badań Materiałowych i Inżynierii Kwantowej, Wydział Inżynierii Materiałowej i Fizyki Technicznej, Politechnika Poznańska | [ P ] employee

Scientific discipline (Law 2.0)

[2.8] Materials engineering

Year of publication

2024

Published in

Analytical and Bioanalytical Chemistry

Journal year: 2024 | Journal volume: vol. 416

Article type

scientific article

Publication language

english

Keywords
EN
  • SERS
  • SERS assay
  • L-Selectin
  • DNA
  • Aptamer
Abstract

EN In the human body, tumor cell occurrence can be indirectly monitored using the L-selectin concentration in the blood, since selectin ligands are present on the surface of tumor cells, and with tumor progression, a decrease in L-selectin levels can be expected and observed. In this study, we present a selective DNA-based surface-enhanced Raman spectroscopy (SERS) assay for the detection and determination of L-selectin in biological samples. Two calibration curves (linear in the 40–190 ng mL−1 region and exponential in the 40–500 ng mL −1 region) are fitted to the obtained SERS experimental data, i.e., the ratio of I732/I1334 band intensities (LOQ = 46 ng mL−1). Calculated determination coefficients are found to be R2 = 0.997 for the linear region of the calibration curve and R2 = 0.977 for the exponential region. Moreover, we demonstrate very good selectivity of the assay even in the presence of P- and E-selectin in a sample containing L-selectin. With our SERS assay, the L-selectin concentration in biological samples can be estimated directly from the calibration curves.

Date of online publication

09.01.2024

Pages (from - to)

1189 - 1197

DOI

10.1007/s00216-023-05110-x

URL

https://link.springer.com/article/10.1007/s00216-023-05110-x

Ministry points / journal

70

Impact Factor

3,8 [List 2023]

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