Development of Thin Film Microextraction with Natural Deep Eutectic Solvents as ‘Eutectosorbents’ for Preconcentration of Popular Sweeteners and Preservatives from Functional Beverages and Flavoured Waters
[ 1 ] Instytut Chemii i Elektrochemii Technicznej, Wydział Technologii Chemicznej, Politechnika Poznańska | [ P ] employee | [ S ] student
2024
scientific article
english
- thin film microextraction
- natural deep eutectic solvent
- aspartame
- acesulfame-K
- parabens
- benzoic acid
- sorbic acid
- energy drinks
- isotonic drinks
EN An eco-friendly method for the determination of sweeteners (aspartame, acesulfame-K) and preservatives (benzoic acid, sorbic acid, methylparaben, ethylparaben) in functional beverages and flavoured waters using thin film microextraction (TFME) and high-performance liquid chromatography with UV detection (HPLC-UV) was proposed. A series of fourteen green and renewable solidified natural deep eutectic solvents (NADESs) were prepared and tested as ‘eutectosorbents’ in TFME for the first time. In the proposed method, the NADES containing acetylcholine chloride and 1-docosanol at a 1:3 molar ratio was finally chosen to coat a support. Four factors, i.e., the mass of the NADES, pH of the samples, extraction time, and desorption time, were tested in the central composite design to select the optimal TFME conditions. Limits of detection were equal to 0.022 μg mL−1 for aspartame, 0.020 μg mL−1 for acesulfame-K, 0.018 μg mL−1 for benzoic acid, 0.026 μg mL−1 for sorbic acid, 0.013 μg mL−1 for methylparaben, and 0.011 μg mL−1 for ethylparaben. Satisfactory extraction recoveries between 82% and 96% were achieved with RSDs lower than 6.1% (intra-day) and 7.4% (inter-day). The proposed ‘eutectosorbent’ presented good stability that enabled effective extractions for 16 cycles with recovery of at least 77%. The proposed NADES-TFME/HPLC-UV method is highly sensitive and selective. However, the use of a solid NADES as a sorbent, synthesized without by-products, without the need for purification, and with good stability on a support with the possibility of reusability increases the ecological benefit of this method. The greenness aspect of the method was evaluated using the Complex modified Green Analytical Procedure Index protocol and is equal to 84/100.
4573-1 - 4573-17
Article number: 4573
CC BY (attribution alone)
open journal
final published version
at the time of publication
public
140
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