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Article

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Title

Microbiological Analysis of Coolant Used in Machining

Authors

[ 1 ] Instytut Technologii Mechanicznej, Wydział Inżynierii Mechanicznej, Politechnika Poznańska | [ P ] employee

Scientific discipline (Law 2.0)

[2.9] Mechanical engineering

Year of publication

2023

Published in

Advances in Science and Technology Research Journal

Journal year: 2023 | Journal volume: vol. 17 | Journal number: iss. 2

Article type

scientific article

Publication language

english

Keywords
EN
  • metalworking fluids
  • CNC machining
  • cooling and lubricating properties
  • microorganisms
Abstract

EN The subject of microbiological tests of coolants presented in the work is the cutting fluid (emulsifying oil) of Fuchs ECOCOOL 68 CF3. The liquid was taken from the tanks of three different numerically controlled machines (Lasertec 65, MillTap 700, CTX beta 1250 TC), used with different intensity. The five most characteristic and popular media were used in the study: nutrient agar - plain, TSA medium (tryptone-soy-agar), medium with dichloran, rose bengal and chloramphenicol, Sabouraud agar with Chloramphenicol, Czapka agar. The incubation time was dependent on the type of microorganisms. After the specified time, the plates inoculated on the medium were subjected to visual observations and under the Leica DM 500 microscope. As a result of the observation, the following types of microorganisms (fungi and bacteria) were detected: Aspergillus Niger Tiegh, Candida Albicans, Saphylococcus Aureus, Micrococcus Luteus and Escherchia Coli and Citrobacter Freundia. The assessments were made in fixed periods. The results are presented as a function of the intensity of the use of cutting fluids. Microbiological analysis of machining fluids will allow for optimizing the time periods of using coolants and will also contribute to the protection of the operator's health and indirectly "extending the life cycle" of the technological machine.

Date of online publication

01.04.2023

Pages (from - to)

206 - 214

DOI

10.12913/22998624/157421

URL

http://www.astrj.com/Microbiological-Analysis-of-Coolant-Used-in-Machining,157421,0,2.html

License type

CC BY (attribution alone)

Open Access Mode

open journal

Open Access Text Version

final published version

Date of Open Access to the publication

at the time of publication

Ministry points / journal

100

Impact Factor

1

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