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Enzymatic membrane reactor in xylose bioconversion with simultaneous cofactor regeneration


[ 1 ] Instytut Technologii i Inżynierii Chemicznej, Wydział Technologii Chemicznej, Politechnika Poznańska | [ 2 ] Instytut Inżynierii Materiałowej, Wydział Inżynierii Materiałowej i Fizyki Technicznej, Politechnika Poznańska | [ D ] phd student | [ P ] employee

Scientific discipline (Law 2.0)

[2.8] Materials engineering
[7.6] Chemical sciences

Year of publication


Published in

Bioorganic Chemistry

Journal year: 2022 | Journal volume: vol. 123

Article type

scientific article

Publication language


  • xylose dehydrogenase
  • xylonic acid
  • enzyme immobilization
  • biomass conversion
  • cofactor regeneration system

EN In this study, we present the concept of co-immobilization of xylose dehydrogenase and alcohol dehydrogenase from Saccharomyces cerevisiae on an XN45 nanofiltration membrane for application in the process of xylose bioconversion to xylonic acid with simultaneous cofactor regeneration and membrane separation of reaction products. During the research, the effectiveness of the co-immobilization of enzymes was confirmed, and changes in the properties of the membrane after the processes were determined. Using the obtained biocatalytic system it was possible to obtain 99% xylonic acid production efficiency under optimal conditions, which were 5 mM xylose, 5 mM formaldehyde, ratio of NAD+:NADH 1:1, and 60 min of reaction. Additionally, the co-immobilization of enzymes made it possible to improve stability of the co-immobilized enzymes and to carry out xylose conversion in six consecutive cycles and after 7 days of storage at 4 °C with over 90% efficiency. The presented data confirm the effectiveness of the co-immobilization, improvement of the stability and reusability of the biocatalysts, and show that the obtained enzymatic system is promising for use in xylose bioconversion and simultaneous regeneration of nicotinamide cofactor.

Date of online publication


Pages (from - to)

105781 - 1 - 105781 - 11





Article Number: 105781

License type

CC BY-NC-ND (attribution - noncommercial - no derivatives)

Ministry points / journal


Impact Factor


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